A Two-Site Model for Reaction Kinetics in the Immunoradiometric Assay (IRMA) of Skeletal Alkaline Phosphatase (sALP)

Abstract

A mathematical model was chosen to fit the antigen-antibody
association kinetics whith a view to distinguishing processes with either one o several binding sites in the reactions involved in the analytical determination of Skeletal Alkaline Phosphatase (sALP) by means of radiometric immunoassay (IRMA). The biexponential model gave the best fit and the most precise estimation of its
parameters. The analysis of the kinetic data conducted
satisfactorily explains the influence of the antigen and antibody concentrations, by admitting that the antigenantibody binding takes place through two different binding site types. This conclusion would have not been reached on the basic of equilibrium data analysis.